Work

Optimizing and Benchmarking Machine Learning and Traditional Synaptic Event Detection Pipelines in Neurophysiology Experiments

Year: 2026

Type: article

Abstract: Synaptic physiology experiments are fundamental to neuroscience research. Consequently, accurate detection of synaptic currents is crucial for conducting high-quality experiments. Traditionally, detecting inhibitory and excitatory postsynaptic currents (sIPSCs/sEPSCs) relied on hand-counting individual events. Although sEPSCs and sIPSCs are clear to the trained eye, hand analysis is time and labor intensive. Recent advances in applied machine learning promise faster, superior event detectors that may improve data quality and reduce or even completely negate the need for hand curation. While many strategies for sIPSC and sEPSC detection exist, rarely have they been quantitatively compared for accuracy within an experiment. Our study aims to establish practical ground-truth event detection in a large experimental dataset through meticulous hand counting and to assess variance in detection results across different laboratories, analysis techniques, and cell types. Using thoroughly hand-counted data as our ground-truth comparison, we benchmark current popular detection methods, including a modern supervised deep learning approach. Our results suggest that current analysis strategies vary widely in their results and that a supervised machine learning approach rivals manual event counting performed by expert electrophysiologists better than other automated approaches.

Source: eNeuro

DOI: 10.1523/eneuro.0410-25.2026