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Preparation, Component Identification, and Antioxidant Activity of Anthocyanins From Punica granatum “Tunisia”

Year: 2026

Type: article

Abstract: ABSTRACT Anthocyanins, a major class of flavonoids with potent antioxidant activity, are abundant in pomegranate ( Punica granatum L.). In this study, anthocyanins were extracted from Punica granatum “Tunisia” and purified to over 70% purity. The major anthocyanin components were characterized by LC–MS/MS analysis. Subsequently network pharmacology and molecular docking suggested that anthocyanins may exert antioxidant effects mainly through the Keap1–Nrf2–HO‐1 signaling pathway. The antioxidant activity of the extract was confirmed by DPPH, ABTS, and FRAP assays. In LPS‐induced RAW264.7 cells, the anthocyanin extract significantly reduced ROS and NO production, enhanced SOD activity, promoted Nrf2 nuclear translocation, downregulated Keap1, and upregulated HO‐1 expression. These results demonstrated that pomegranate anthocyanins exhibited strong antioxidant activity and may serve as promising natural antioxidants for functional food applications.

Source: AgriFood Journal of Agricultural Products for Food

DOI: 10.1002/agf2.70017

Authors: Jing Zhang, Xu‐han Yang, Meng‐qi Zhang, Xu Guo, Ning‐yang Li, Fang‐yu Long, Chao Liu

Institutions: Shandong Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Ocean University of China

Language: English

Topic: Pomegranate: compositions and health benefits

Subfield: Nutrition and Dietetics

Field: Nursing

Domain: Health Sciences

SDGs: Good health and well-being, Zero hunger

Open Access status: hybrid

OpenAlex ID: W7154696141

FWCI:

Cites: 31

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