Combined application of a GSK3 inhibitor and injectable platelet rich fibrin with deproteinized bovine bone improves bone regeneration
Year: 2026
Type: article
Abstract: This preliminary experimental study aimed to explore the osteoregenerative capacity of a novel combinatorial approach integrating deproteinized bovine bone (DBB), the glycogen synthase kinase-3 (GSK-3) inhibitor Tideglusib, and injectable platelet-rich fibrin (I-PRF) in a rat critical-sized calvarial defect model. A total of 36 adult rats (250–350 g) were included, comprising four donor animals and 32 experimental subjects. Standardized critical-sized defects were surgically created in the parietal bone of each rat. The animals were randomly assigned to four groups (n = 8 per group): DBB alone (control), DBB + Tideglusib, DBB + I-PRF, and DBB + Tideglusib + I-PRF. Injectable PRF was prepared from intracardiac blood collected from donor rats and incorporated into the designated grafting protocols. Following an 8-week healing period, the animals were euthanized, and the calvarial specimens were harvested for histomorphometric quantification of newly formed bone. Statistical analysis revealed significant differences in regenerative outcomes between the DBB group and the other groups, with the highest extent of new bone formation observed in defects treated with the triple-combination therapy. The findings of this preliminary study suggest that the combined use of DBB, Tideglusib, and I-PRF may represent a promising grafting strategy for enhancing bone regeneration.
Source: Scientific Reports
Authors: Mehmet Onur Merey, Göksel Şimşek Kaya, Mahir Kaya, Selen Elif Merey, Büşra Altunay, Havva Serap Toru
Institutions: Akdeniz University, Aksaray University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,690
OpenAlex ID: W7154703106