Translational strategy to support the first-in-human study of a TCR-like T cell bispecific with an in vitro-based safety approach
Year: 2026
Type: article
Abstract: The nonclinical development of T-cell receptor (TCR)–based therapeutics is uniquely challenged by the human-exclusive specificity of the peptide-HLA target, rendering conventional animal models unsuitable for safety assessment. To support first-in-human (FIH) studies in patients with solid tumors with the novel TCR-like T Cell Bispecific (TCB) antibody MAGE-A4-TCB, targeting the MAGE-A4 peptide presented by HLA-A*02:01 and engaging T-cells via the CD3ϵ chain, we implemented a comprehensive, prediction-independent safety strategy relying on New Approach Methodologies (NAMs). This approach utilized 21 human cellular systems, including 2D and 3D in vitro models of vital organs (such as liver, heart, kidney, and lung), co-cultured with allogeneic peripheral blood mononuclear cells to identify potential off-target toxicity and a whole blood assay for cytokine release risk assessment. The concentration-effect relationship and Minimal Anticipated Biological Effect Level (MABEL) were established using the A375 cancer cell line, chosen for its comparable sensitivity to ovarian carcinoma organoids, which were the most sensitive out of 22 tested tumor organoids. Nonclinical testing demonstrated an absence of off-target reactivity in vital organs, though the whole blood assay predicted cytokine release syndrome as the primary risk. The calculated FIH starting dose was safe in the subsequent Phase I study (NCT05129280), and the most commonly reported adverse events were treatment-related skin and subcutaneous tissue disorders (73% of patients). These skin-related adverse events, which appeared in contradiction to the initial in vitro results in skin co-culture models, were hypothesized to be driven by the target-independent activation capability of the UCHT1-based CD3 binder, potentially enhanced in patients with a systemic pro-inflammatory profile. This study provides a translational framework for TCR-based immunotherapies, demonstrating the successful application of NAMs to define a safe starting dose while highlighting the critical need for refining these methodologies to accurately predict complex, systemic immune-related adverse events, particularly those involving barrier organs.
Source: Frontiers in Immunology
Authors: Angelique Augustin, Blandine Avignon, Christophe Boetsch, Ekaterina Breous-Nystrom, Olaf Broders, Lauriane Cabon, Karen Dernick, Evelyne Durr, Miro Julian Eigenmann, Susanne Fischer, Nick Flinn, Regine Gerard, Anna Maria Giusti, Nikolche Gjorevski, Hans Juergen Grote, Fabian Häusermann, Nina Hobi, Elisabeth Husar, Laurent Juglair, Simon Patrick Keiser, Nino Keshelava, Stefan Kustermann, Celine Marban-Doran, Estelle Marrer-Berger, Iris Martinez Quetglas, Virginie Micallef, Ramona Matheis, Daniela Ortiz Franyuti, Liudmila Polonchuk, Giulia Raggi, Andreas Roller, Pamela Ruffiner, Salma Sadok, Tulun Saylan, Nathalie Schaub, Desirée Schubert, Satyajit Shetage, Nadine Stokar-Regenscheit, Antje-Christine Walz, Tina Weinzierl, Vincent Wolowski, Christine Zihlmann
Institution: Roche (Switzerland)
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W7154742312