Cervical cancer screening - comparison between molecular assay to detect Human Papilloma Virus (HPV) DNA genotype and cytology examination from LBC samples in a Reference Laboratory in East India
Year: 2026
Type: article
Study design: Observational Study
Abstract: Cervical cancer is a public health problem in developing countries like India, so much so that India alone accounts for one-quarter of the worldwide burden of cervical cancers; and Human papilloma virus (HPV) is the most common causative factor. 30 different strains of HPV is associated with high risk for cervical cancer. The premise of our descriptive prospective study is based on the above context and compares HPV genotype prevalence with cytological findings. Cervical samples were collected in Eziprep liquid based cytology vials from 1009 patients from East/ North East India. HPV DNA genotyping was done using automated platform of QiaSymphony SP and cytology smears were made using Eziprep Nanocyt machine which uses Thin prep type LBC method. Commonest genotypes detected were 16 and 52, both of which were high risk genotypes. Cases with multiple infections also had at least one high risk genotype. 17 showed epithelial abnormality and most common type was atypical squamous cell of uncertain significance (ASCUS) followed by Atypical squamous cell cannot rule out high grade lesion (ASC-H). Most common genotypes associated with epithelial abnormalities were 16 followed by 58 and 59. 3 cases were identified with HPV negative epithelial abnormality. HPV 16 was found to be most common high risk genotype. Contrary to literature, 2nd most common genotype was not found to be HPV 18. HPV 52 was overall 2nd most common genotype while 58 and 59 were 2nd most common genotypes associated with epithelial abnormality.
Institution: Dr. Lal PathLabs Pvt Ltd (India)
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7154916398