Work

Molecular Probes for Tracking Reactive Oxygen Species at the Organelle Interface

Year: 2026

Type: article

Abstract: The spatially compartmentalized production and regulation of reactive oxygen species (ROS) play pivotal roles in cellular physiology and pathology. Over the past two decades, significant technological advances have propelled the development of fluorescent molecular probes and genetically encoded sensors to monitor redox dynamics within distinct organelles. This perspective review discusses recent progress and associated challenges in ROS tracking at the organelle interface, with particular emphasis on mitochondria, the endoplasmic reticulum (ER), lysosomes, and the emerging frontier of peroxisomal imaging. We examine chemical and protein‐based probe design strategies, target specificity issues, and the integration of multiplexed imaging techniques. Particular attention is given to the prospects for peroxisomal ROS tracking, a compartment historically underexplored despite its critical role in lipid metabolism and redox regulation and offer perspectives on how novel probe engineering may overcome current limitations. The ongoing evolution of these imaging modalities promises to deepen our understanding of subcellular redox signaling in both physiological and pathological states.

Source: Analysis & Sensing

DOI: 10.1002/anse.202600003

Authors: Daniela Ceballos‐Ávila, Arturo Jiménez‐Sánchez

Institution: Universidad Nacional Autónoma de México

Language: English

Topic: Molecular Sensors and Ion Detection

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,840

Funder: Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México