Work

Optimization of Process Conditions for the Separation and Purification of Erythromycin Thiocyanate Using Response Surface Methodology

Year: 2026

Type: article

Abstract: A water acetone biphasic extraction system was developed for the separation and purification of erythromycin thiocyanate. Response surface methodology based on a Box-Behnken design was used to evaluate the effects of pH, liquid-to-solid ratio, extraction temperature, and acetone-to-water volume ratio on mass yield. All four variables influenced the extraction performance, and acetone-to-water volume ratio and liquid-to-solid ratio were the most significant factors. Under the optimized conditions of 50.5 °C, pH 9.2, a liquid-to-solid ratio of 3.0 mL/g, and an acetone-to-water volume ratio of 2.5 mL/mL, the mass yield reached 81.58 percent. The predicted and experimental values were in good agreement, confirming the adequacy of the model. The product obtained under the optimized conditions met the relevant requirements of the Chinese Pharmacopoeia. The proposed process is simple and effective, and provides a basis for the purification and scale up of erythromycin thiocyanate and related derivatives.

Source: Separations

DOI: 10.3390/separations13040126

Authors: Yining Wang, Yinghua Sun, Wuying Li, Shuqian Xia

Institution: Tianjin University

Language: English

Topic: Chemical and Physical Properties in Aqueous Solutions

Subfield: Filtration and Separation

Field: Chemical Engineering

Domain: Physical Sciences

SDG: Responsible consumption and production

Open Access status: gold

Estimated APC paid: $3,137