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Advanced NMR Characterization and Sensitive Detection of Isoaspartate in Proteins

Year: 2026

Type: article

Abstract: H-only NMR, enabling the detection of 10 nmol of isoAsp-containing protein in 1 h. We exemplify this approach on therapeutic products like insulin or the monoclonal antibody trastuzumab.

Source: Analytical Chemistry

DOI: 10.1021/acs.analchem.5c07290

Authors: Manon Julien, Sophie Zinn-Justin, Francois-Xavier Theillet

Institutions: Centre National de la Recherche Scientifique, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Université Paris-Saclay, Institut de Biologie Intégrative de la Cellule, Université Paris Cité, Cibles Thérapeutiques et conception de médicaments

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,500

Funders: Agence Nationale de la Recherche, Centre National de la Recherche Scientifique, Commissariat à l'Énergie Atomique et aux Énergies Alternatives

Awards: Regulation of the DNA-damage response by phosphorylation clusters in the p53 signaling network, DNA damage signaling studied by in-cell NMR: Structural and functional regulation of Mdm2 through its phosphorylation status.

OpenAlex ID: W7155105364

FWCI:

Cites: 60

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