Quantitative Spatial Profiling of Antibody Delivery and Target Engagement Using Optically Labeled Antibodies
Year: 2026
Type: article
Abstract: Visualization of drug distribution in human tumors is critical for optimizing biologic therapeutics, yet current clinical workflows cannot colocalize drugs and biomarkers on the same formalin-fixed, paraffin-embedded section. Methods: We present a reproducible workflow that integrates near-infrared imaging of optically labeled therapeutic antibodies with multiplex immunostaining and computational registration to enable quantitative, single-cell analysis. Results: Using panitumumab–IRDye800CW, we demonstrated heterogeneous accumulation in epidermal growth factor receptor–positive regions and quantified penetration gradients relative to tumor architecture. Conclusion: We established a multiplex imaging platform that enables single-cell analysis of therapeutic antibody delivery and pharmacodynamics in human formalin-fixed, paraffin-embedded tissues.
Source: Journal of Nuclear Medicine
Institutions: Vanderbilt University Medical Center, Vanderbilt University
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7155186515