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From raw data to processed spectra: A step-by-step guide

Year: 2026
Type: article
Abstract: Optical spectroscopy is an important and widely used technique, for instance, to characterize new materials and to identify unknown compounds. Spectra are typically reported as a function of the wavelength of light, yet the information extracted from such spectra can be misleading. In contrast, spectra represented as a function of the frequency (or photon energy) allow for a more direct extraction of the intrinsic quantum-mechanical properties of the materials under investigation. Here, we discuss this conversion for absorption, fluorescence, and fluorescence excitation spectra. We show step-by-step the different factors that lead to a rescaling of the measured absorption and fluorescence signals.
Language: English
Open Access status: hybrid
OpenAlex ID: W7155209171
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Cites: 13
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