Environmental dissemination of multidrug-resistant Escherichia coli across one health interfaces in Mymensingh, Bangladesh
Year: 2026
Type: article
Abstract: Environmental dissemination of antimicrobial-resistant Escherichia coli is a major One Health challenge in Bangladesh. This study assessed the occurrence of third-generation cephalosporin and carbapenem-resistant E. coli across key environmental interfaces in Mymensingh. In May 2022, 28 water samples were collected from hospital wastewater, livestock effluents, aquaculture ponds, and the Khiro River. Samples were processed via the WHO Tricycle protocol, with 26 isolates undergoing whole-genome sequencing. Third-generation cephalosporin-resistant E. coli were detected in 86% of samples, while carbapenem-resistant isolates (18%) were found exclusively in hospital and river samples. Highest ESBL-producing E. coli concentrations occurred in hospital and poultry wastewater (mean 6.9-7.1 log10 CFU/ml). Sequencing identified 93 resistance genes, dominated by blaCTX-M-15 (79%), tet(A) (75%), aadA1 (54%), qnrS1 (50%), and mph(A) (50%). Carbapenem resistance was associated with blaNDM-5 in hospital and river isolates. While most isolates showed niche-specific clustering in our Phylogenetic analysis, highly conserved core-genome profiles (0 SNPs) between hospital and downstream river isolates (ST2363/ST410) provided strong genomic evidence consistent with effluent-mediated dissemination. These findings highlight the role of wastewater and livestock systems in AMR transmission, underscoring the urgent need for integrated One Health surveillance and improved waste management in Bangladesh.
Authors: Aninda Rahman, Sangjukta Roy, Nawroz Afreen, Mohammad Gazi Shah Alam, Mohammad Sadekuzzaman, Abul Kalam, Faisol Talukdar, Md. Barkatul Alam, Md. Shafiul Alam, S. M. Sabrina Yesmin, Anders Rhod Larsen, Lina M. Cavaco, Andreas Petersen, Kazi Injamamul Hoque, Mohammed Badrul Amin, Rezaul Hasan, Johanna Lindahl, Jérôme Delamare-Deboutteville, Arshnee Moodley
Institutions: Directorate General of Health Services, Ministry of Health and Family Welfare, Mymensingh Medical College Hospital, Institute of Epidemiology, Disease Control and Research, University of Washington, Department of Livestock Services, Chattagram Maa-O-Shishu Hospital Medical College, Department of Fisheries of the Government of the People's Republic of Bangladesh, Statens Serum Institut, International Centre for Diarrhoeal Disease Research, Griffith University, International Livestock Research Institute, WorldFish, University of Copenhagen
Language: English
Subfield: Pollution
Field: Environmental Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,190
OpenAlex ID: W7156284956