Unraveling the mechanisms of pseudouridylation in rice plant immunity: The power of next-generation sequencing
Year: 2026
Type: article
Abstract: ABSTRACT Pseudouridylation is a prevalent RNA modification that plays a pivotal role in regulating gene expression and modulating plant responses to biotic stresses, such as pathogenic attacks. Recent advancements in next-generation sequencing technologies have enabled high-resolution mapping of pseudouridine sites in plant RNA, offering valuable insights into the molecular mechanisms underlying plant immunity. This review explores the role of pseudouridylation in rice under biotic stress conditions, particularly during interactions with fungal, bacterial, and viral pathogens. We highlight the potential of NGS-based methods, such as RNA-seq and pseudouridine-seq, in identifying and quantifying pseudouridylation marks in response to pathogen-induced stress. The review further discusses the regulatory implications of pseudouridylation in modulating gene expression, post-transcriptional control, and host-pathogen interactions. Understanding how pseudouridylation influences plant immunity opens new avenues for enhancing disease resistance in rice and other crops. We also consider the challenges and future prospects of integrating NGS-based technologies in plant pathology research to unravel the complexities of RNA modifications in response to biotic stress.
Source: Current Plant Biology
Authors: Misbah Naz, Xiaosi Bu, Muhammad Rahil Afzal, Zhuo Chen
Institution: Guizhou University
Language: English
Topic: RNA modifications and cancer
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $3,440
OpenAlex ID: W7156378540