Work

One‐Pot RAA ‐ CRISPR /Cas12a Assay for Rapid Detection of Infectious Hypodermal and Haematopoietic Necrosis Virus ( IHHNV ) in Shrimp Aquaculture

Year: 2026

Type: article

Abstract: The infectious hypodermal and haematopoietic necrosis virus (IHHNV) represents a significant viral threat to global shrimp aquaculture, leading to considerable economic losses. In this study, we have developed a one-step, one-pot isothermal assay for the detection of IHHNV, employing recombinase-aided amplification in conjunction with clustered regularly interspaced short palindromic repeats-Cas12a (RAA-CRISPR/Cas12a). The assay is performed at a constant temperature of 37°C, achieving a detection limit of 10 copies per reaction for the fluorescence assay and 1 copy per reaction for the lateral flow dipstick (LFD) assay within a 60-min timeframe. Additionally, we evaluated the assay against four other prevalent shrimp pathogens (WSSV, DIV1, EHP, VpAHPND) and observed no cross-reactivity. This straightforward detection method exhibits high sensitivity and specificity for IHHNV, offering a promising approach for early and rapid field diagnosis.

Source: Journal of Fish Diseases

DOI: 10.1111/jfd.70196

Authors: Zewei Hou, Yichen Zhao, Zheng Sun, Ying Zhan, Xilin Dai, Hao Wang

Institution: Shanghai Ocean University

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDGs: Zero hunger, Good health and well-being

Open Access status: green

OpenAlex ID: W7159580133