In vitro Propagation and Foliar Micromorphological Studies of Dillenia indica L.: An Underutilized Fruit Tree
Year: 2026
Type: article
Abstract: The present study describes an improved, efficient method for in vitro propagation of the underutilized fruit tree, Dillenia indica L. Nodal segments obtained from a mature tree were used as initial explants for in vitro regeneration. Among the various cytokinin types (BAP, Kin, m T, and TDZ) and concentrations tested, MS medium containing 0.5 mg·L −1 6-benzylaminopurine was optimal for shoot induction. Subculturing mother explants into fresh medium during subsequent passages significantly influenced shoot multiplication. A maximum of 5–7 shoots were regenerated after the third subculturing on MS medium containing 0.5 mg·L −1 6-benzylaminopurine. Maximum rooting was achieved in MS medium containing 2 mg·L −1 indole-3-butyric acid. Approximately 80% of the plants survived acclimatization. A comparative study revealed significant changes in leaf micromorphology and architecture, including developmental and structural changes in venation patterns, trichomes, and stomata, in both in vitro -grown and fully acclimatized plants. This foliar micromorphological study elucidated the mechanism of adaptation of in vitro -regenerated plantlets during acclimatization. The current investigation could provide a foundation for the large-scale propagation and conservation of this medicinally important and underutilized fruit tree species.
Authors: Amit Singh, Srishti Yadav, Manoj K. Rai
Institution: Indira Gandhi National Tribal University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $323
OpenAlex ID: W7160020809