Work

Nuclear Galectin-1 Drives Cancer Progression through O -GlcNAcylation-Dependent Regulation of SOX2

Year: 2026

Type: article

Abstract: Galectin-1 is frequently upregulated in tumors and contributes to cancer progression.Here, we identify galectin-1 as a critical regulator of cancer stem-like properties.Silencing galectin-1 suppressed proliferation, motility, side population fraction, and tumorsphere formation in vitro, and impaired tumor initiation and growth in vivo, whereas overexpression enhanced these malignant phenotypes.Transcriptomic profiling revealed stemness-associated transcription factors as major downstream targets, with SOX2 emerging as a key effector.Galectin-1 knockdown reduced SOX2 expression, whereas overexpression increased SOX2 nuclear abundance and transcriptional activity.Rescue experiments demonstrated that SOX2 is functionally required for galectin-1-mediated stemness and tumorigenesis.Mechanistically, galectin-1 associates with SOX2 in an O-GlcNAcylation-dependent manner.Inhibition of O-GlcNAcylation or mutation of SOX2 O-GlcNAc sites disrupted this interaction, reduced SOX2 transcriptional activity, and impaired tumorsphere formation, supporting an intracellular lectin-like function.Structural modeling predicted that residues E71 and R73 within the carbohydrate recognition domain are critical for carbohydrate-mediated recognition of O-GlcNAc-modified SOX2, which was validated by mutagenesis.Clinically, galectin-1 was highly expressed in gastric tumors, correlated with advanced stage, and predicted poor prognosis.Notably, high co-expression of galectin-1 and SOX2 was significantly associated with unfavorable survival outcomes.These findings establish galectin-1 as a reader-like protein that functionally engages O-GlcNAcylated SOX2 and highlight the galectin-1/SOX2 axis as a potential therapeutic target in gastric cancer.

Source: International Journal of Biological Sciences

DOI: 10.7150/ijbs.124928