Work

A guide to selecting high-performing antibodies for SCARB2 (UniProt ID: Q14108) for use in western blot, immunoprecipitation, and immunofluorescence

Year: 2026

Type: article

Abstract: Scavenger receptor class B member 2 (SCARB2), also known as lysosomal integral membrane protein type 2 (LIMP-2), is a multifunctional glycoprotein critical for lysosomal biogenesis and neuronal homeostasis. We characterized twelve commercially antibodies against SCARB2 in western blot, immunoprecipitation, and ten in immunofluorescence using a standardized workflow. Antibody performance was assessed by comparing signals in a wild-type cell line and its corresponding knockout derivative. This work is part of a broader collaborative public-good initiative to improve biomedical research by systematically evaluating commercial antibodies against human proteins and openly sharing the data as a resource for the scientific community. We encourage readers to use this report as a guide for selecting antibodies best suited to their specific applications.

Source: F1000Research

DOI: 10.12688/f1000research.178929.1