SUMO-assisted expression of a soluble and functional Venus flytrap domain of the human sweet taste receptor TAS1R2 in Escherichia coli
Year: 2026
Type: article
Abstract: The heterodimeric human sweet taste receptor TAS1R2/TAS1R3 is a class C G-protein-coupled receptor responsible for detecting a wide range of sweet-tasting compounds. The Venus flytrap domain of hTAS1R2 (hTAS1R2-VFT) constitutes the main binding site for natural sugars and some noncaloric sweeteners, including sucralose, neotame, and acesulfame-K. However, its biophysical characterization has been limited by difficulties in producing soluble and functional protein, with most strategies relying on refolding from inclusions bodies. In this study, we report the successful expression of soluble, folded, and functional hTAS1R2-VFT using Escherichia coli and a N-terminal small ubiquitin-like modifier (SUMO) fusion protein, avoiding protein refolding from inclusion bodies. A two-step purification process (immobilized metal ion affinity chromatography followed by preparative gel filtration) yielded approximately 0.42 mg of pure protein per liter of culture. Circular dichroism spectroscopy and size-exclusion chromatography coupled with multiangle static light scattering analysis confirmed proper folding and monomeric behavior of SUMO-hTAS1R2-VFT. Functional characterization using intrinsic tryptophan fluorescence revealed that SUMO-hTAS1R2-VFT bound sweet-tasting compounds with affinities consistent with their physiological relevance. Neotame exhibited the highest affinity ( K d ≈ 2 μM), followed by acesulfame-K ( K d ≈ 116 μM) and sucralose ( K d ≈ 282 μM), while sucrose showed weak binding in the millimolar range. These affinities were in agreement with sweetness potency and comparable to those reported for the full-length TAS1R2 subunit. This work provides the first demonstration of soluble expression of functional hTAS1R2-VFT in bacteria, by adding a solubility tag, offering a robust and scalable platform for studying sweetener-receptor interactions and facilitating the rational design of novel sweeteners.
Language: English
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,680
OpenAlex ID: W7160208955