Work

Hydrocephalus caused by Katnip deletion is linked to increased ciliogenesis and reduced proliferation of neuroprogenitor cells

Year: 2026

Type: preprint

Abstract: Background KATNIP (Katanin-interacting protein), also known as KIAA0556 , is one of the human genes with pathogenic variants linked to Joubert syndrome, an archetypal neurodevelopmental ciliopathy. KATNIP is a scaffolding protein with a critical role in ciliogenesis. In this study, we characterized the ciliopathy phenotypes due to KATNIP gene deletion. Results We produced a Katnip null mouse model using CRISPR-Cas12a (Cpf1). The null heterozygotes appeared normal while the homozygotes died around postnatal day 9, showing severe hydrocephalus and deficiency in neuroprogenitor cell proliferation. Katnip-deficient cells in the brain have a higher rate of cilia formation and longer cilia than wild type cells. Conclusion KATNIP loss of function gives rise to hydrocephalus found in Joubert syndrome. The results indicate that KATNIP restricts ciliogenesis and cilia extension and supports proliferation of neuroprogenitor cells in the brain.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.64898/2026.05.01.722314

Authors: Ana Limerick, Cheuk Ying Chu, Jacob S. Turner, David L. Brautigan, Wenhao Xu, Zheng Fu

Institution: University of Virginia

Language: English

Topic: Genetic and Kidney Cyst Diseases

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences