Work

Deep eutectic solvent tailoring the electrode-enzyme interface for phenylalanine dehydrogenase immobilization and efficient bioelectrosynthesis

Year: 2026

Type: article

Abstract: Deep eutectic solvents (DESs) are a group of emerging solvents for biocatalysis with tailored performance. Herein, we report the usage of hierarchical zeolitic imidazole framework-8 (HZIF-8) and halogenated DES for the immobilization of phenylalanine dehydrogenase. Effects of three different halogenated DESs (I, Cl and Br) on the activity of immobilized enzymes for production of homophenylalanine were studied. The optimal activity towards with the model reaction of catalyzing ethyl 2-oxo-4-phenylbutyrate (EOPB) to L -homophenylalanine was 6.11 U/mg at 70 °C, 5.4-fold of that of the free enzyme, with 78.4% of its original activity retained after six recycles. The immobilized enzymes were further loaded on a poly(methylene blue) electrode with electrochemical NAD + regeneration, leading to bioelectrosynthesis of phenylpyruvate. Using phenylalanine-ethanolamine (Phe-MEA) as the substrate, 13.85 mM phenylpyruvate was obtained after 8 h, with a total turnover number (TTN) value of 138.5 for NADH regeneration, while a TTN of 102.2 using L-Phe as the substrate.

Source: Bioelectrochemistry

DOI: 10.1016/j.bioelechem.2026.109315

Authors: Jintian Zhang, Feixuan Li, Xiaoyan Xiang, Xinxin Xiao, Shizhen Wang

Institutions: Xiamen University, Aalborg University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Affordable and clean energy

Open Access status: hybrid

Estimated APC paid: $3,970