Work

Regulation of chromatin dynamics by a calcium-dependent nucleoskeleton

Year: 2026

Type: article

Abstract: Growth factors selectively activate calcium signaling pathways in the cell nucleus, which in turn regulate gene transcription and other intranuclear events, but the specific way this is accomplished is not entirely understood. Growth factors increase inositol 1,4,5-trisphosphate (IP3) in the nucleus, which in turn releases calcium from intranuclear IP3 receptors, and the present study shows that this leads to transient assembly of an actin nucleoskeleton that associates with intranuclear nonmuscle myosin 2A (MYH9). Mass spectrometry suggests that much of the MYH9 cargo consists of components of the gene transcription machinery, and chromatin immunoprecipitation identified a number of specific genes that associate with the myosin in response to stimulation with growth factors. Together, these findings suggest that growth factors initiate gene transcription by transiently assembling an actin nucleoskeleton that works with MYH9 to bring specific genes to the transcription machinery.

Source: Journal of Biological Chemistry

DOI: 10.1016/j.jbc.2026.113104

Authors: María José González, Michele Angela Rodrigues, Santo Diprima, Dejian Zhao, Thais Fernandes Bassani, Clara Couto Fernandez, Emma Kruglov, Michael H. Nathanson, Dawidson Assis Gomes

Institutions: Universidade Federal de Minas Gerais, Yale University

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,430

Funders: Yale University, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Canadian Agri-Food Policy Institute, National Institutes of Health

Awards: G1049258176, G1521275299, DESVENDANDO A VIA DOS RECEPTORES DE INOSITOL 1,4,5-TRIFOSFATO ISOFORMA 3 (ITPR3) NA FORMAÇÃO DE FILAMENTOS DE ACTINA NO NÚCLEO: UMA NOVA VIA COM POTENCIAL TERANÓSTICO PARA O CÂNCER., A swept-field confocal microscope for live cell imaging, G638115972, ESTUDO DA FUNÇÃO DA TRANSLOCAÇÃO DO RECEPTOR DO FATOR DE CRESCIMENTO EPIDERMAL (EGFR) PARA O NÚCLEO PARA ATIVAÇÃO DA NOVA FOSFOLIPASE DELTA 4 (PLCD4) NUCLEAR NA PROLIFERAÇÃO DE CÉLULAS-TRONCO MESENQUIMAIS INICIADORAS DE TUMOR, G8656719847, G9000007135, G9000010113, G9000017845, G9000102924, G9000213485, ITPR3 and aberrant mitochondrial calcium signaling in alcoholic hepatitis, Role of Neutrophils in the Pathogenesis of Cholangiopathies

OpenAlex ID: W7160423297

FWCI: 3.877

Cites: 36

Cited by: 2

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