Work

Cuevaenes F–H, Including Two Rare Chlorinated Cuevaenes From the Marine‐Derived Streptomyces malaysiensis HNM0561

Year: 2026

Type: article

Abstract: ABSTRACT Three new cuevaenes, designated cuevaenes F–H ( 1 – 3 ), together with four known analogues ( 4 – 7 ), were isolated from the marine‐derived strain Streptomyces malaysiensis HNM0561. Their structures and absolute configurations were elucidated using HR‐ESIMS, extensive 1D and 2D NMR spectroscopy, and ECD calculations. Notably, this study establishes the absolute configurations of previously reported cuevaenes ( 4 , 5 , and 7 ) for the first time. Compounds 1 and 2 are distinguished by a rare chlorine substitution at C‐17, representing a novel structural motif within the cuevaene family. Additionally, compounds 1 – 6 were derivatized via esterification with (trimethylsilyl)diazomethane (TMSCHN 2 ) to afford the corresponding methyl esters ( 1a – 6a ). All isolated and derivatized compounds ( 1 – 7 and 1a – 6a ) were evaluated for their inhibitory effects on nitric oxide (NO) production in lipopolysaccharide (LPS)‐activated BV2 microglial cells. Among them, compounds 3 and 4a exhibited significant inhibitory activity, reducing NO production by 54% and 47% at a concentration of 20 µM, respectively.

Source: Chemistry & Biodiversity

DOI: 10.1002/cbdv.71279