Work

Design, synthesis, and multitarget evaluation of thiosemicarbazone–sulfonamide hybrids as potent cholinesterase and MAO-A inhibitors with neuroblastoma-associated cytotoxicity

Year: 2026

Type: article

Abstract: Neurodegenerative disorders and neuroblastoma represent major therapeutic challenges, and multitarget approaches have gained increasing attention. In this study, a series of thiosemicarbazone derivatives (5a–t) was evaluated for their inhibitory activity against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and monoamine oxidase A (MAO-A), together with their cytotoxic effects and molecular interaction profiles. In vitro enzyme assays revealed nanomolar inhibition for several compounds. Notably, compound 5n exhibited potent and balanced multitarget activity with IC 50 values of 104.28 nM (AChE), 23.04 nM (BChE), and 215.50 nM (MAO-A), surpassing galantamine (IC 50 = 296.32 and 105.20 nM for AChE and BChE, respectively) and approaching the activity of clorgyline (IC 50 = 401.68 nM). Kinetic studies confirmed strong enzyme binding, with K i values of 92.42 nM (AChE) and 25.35 nM (BChE). Cytotoxicity assays against SH-SY5Y neuroblastoma cells showed selective antiproliferative effects, with compound 5n displaying an IC 50 of 5.23 µM and a selectivity index of 8.6 relative to HUVEC cells. Molecular docking and MM-GBSA analyses revealed strong binding affinities (docking scores − 10.4 to − 15.4 kcal/mol; ΔG_bind − 55.9 to − 88.4 kcal/mol), which were further supported by molecular dynamics simulations. DFT calculations indicated favorable electronic properties (HOMO–LUMO gap: 0.098–0.116 eV), while ADME predictions suggested acceptable drug-like behavior and good oral absorption. These results identify thiosemicarbazone derivative 5n as a promising multitarget lead for the development of agents targeting cholinergic dysfunction, MAO-A inhibition, and neuroblastoma proliferation.

Source: Scientific Reports

DOI: 10.1038/s41598-026-52909-6