Monitoring the infectious titer of bOPV and the vaccine vial monitor stage at different storage temperature ranges
Year: 2026
Type: article
Abstract: Poor storage of the polio vaccine in areas of inadequate or absent power to sustain the cold chain can compromise its potency. This study assessed the sustainability of bivalent Oral Polio vaccine (bOPV) potency using total viral titer and the color of the vaccine vial monitor (VVM) at different storage temperatures over time. Weekly vaccine titers were obtained until the VVM degraded to 100%. Fifty vials of bOPV tested before exposure had titers above 107.0 CCID50/mL (≥106.0 CCID50/0.1 mL), and VVM was rated at 0% degradation. At 2–4°C, 100% of the vaccine samples sustained potency for 6 months, and the color of VVM remained at 0% degradation after 3 months, but 10%, 20%, 20% after 4, 5, and 6 months, respectively. At 27–29°C, all the vaccine samples sustained potency at 1 week, 50% after 2 weeks, and their VVM were rated 10%, 20%, 40% and 100% after 1, 2, 3, and 4 weeks, respectively. The potency of the 50% of vaccine samples stored at 35–36°C remained stable for a week, with their VVM rated 10%, 50%, and 100% after storage for 1, 2, and 3 weeks, respectively. At atmospheric temperatures (22–44°C), the titers of all the vaccine samples dropped significantly, and VVM attained 100% degradation within a week. The potency of the bOPV was preserved under refrigeration for 6 months, at room temperature for 2 weeks, and at an incubator for a week and severely degraded at atmospheric temperatures within a week.
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,175
OpenAlex ID: W7160828621