Work

Data from: Spatial and longitudinal tracking of enhancer-AAV vectors that target transgene expression to injured mouse myocardium

Year: 2025

Type: dataset

Abstract: Here, we applied in vivo bioluminescent imaging to mice systemically injected with AAV vectors containing different combinations of capsids, enhancers, and timing of delivery. Longitudinal tracking of expression directed by different TREEs revealed distinct amplitudes and durations of reporter gene expression in the injured heart. The liver-de-targeted AAV capsid, AAV.cc84, could deliver TREEs either pre- or post-cardiac injury to negate off-target expression in the liver while maintaining transduction in the heart. By screening AAV9-based capsid libraries dosed systemically in mice post-cardiac injury, we discovered a new capsid variant, AAV.IR41, with enhanced transduction in cardiac injuries and with elevated transduction of TREE driven transgenes versus conventional AAV9 vectors. Datasets include images, image analyses, sequencing results, spreadsheets, and statistical analyses.

Source: DRYAD

DOI: 10.5061/dryad.2547d7x4p

Authors: Wolfson, David, Hull, Joshua, Li, Yongwu, Gonzalez, Trevor, Jayaram, Mourya, Devlin, Garth, Cigliola, Valentina, Oonk, Kelsey, Rosales, Alan, Bursac, Nenad, Asokan, Aravind, Poss, Kenneth

Institutions: Duke University, Vanderbilt University, University of Wisconsin–Madison, Morgridge Institute for Research

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Heart, Lung, and Blood Institute, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Biomedical Imaging and Bioengineering

OpenAlex ID: W7160947085

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