Chemiluminescent enzyme-linked immunosorbent assay using monoclonal antibodies for zearalenone in food and feed
Year: 2026
Type: article
Abstract: A chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for the detection of zearalenone (ZEN) in maize and feed was developed by optimising antigen and antibody concentrations, as well as incubation times. The results indicated that the CL-ELISA exhibited a linear working range of 0.039–1.027 ng mL −1 , with a half-maximal inhibitory concentration (IC 50 ) of 0.187 ng mL −1 and a limit of detection (LOD) of 0.039 ng mL −1 . The method exhibited cross-reactivity ranging from 7.95 to 102.19% for five ZEN structural analogues, with no detectable cross-reactivity toward other mycotoxins or the carrier protein. The intra- and inter-assay coefficients of variation were both below 10% and recovery rates in spiked maize and feed samples ranged from 86.68 to 103.86%. In conclusion, the developed CL-ELISA is suitable for the rapid, group-specific screening of ZEN in maize and feed.
Source: Acta Alimentaria
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
OpenAlex ID: W7161148306