Additional file 3: Figure S2. of EPCR promotes breast cancer progression by altering SPOCK1/testican 1-mediated 3D growth
Year: 2017
Type: article
Abstract: Effects of EPCR silencing in vitro and in vivo tumor growth in an orthotopic model. A. Western blot analysis of EPCR protein levels in human BT-549 (top) and murine ANV5 (bottom) cells transduced with a scramble shRNA (shControl) and shRNAs targeting human (shEPCR#1 and shEPCR#2 in BT-549) and murine (shEPCR#3 in ANV5) EPCR. β-tubulin was used as loading control. White line indicates that the membrane was cut. B. MTS in vitro proliferation assay of BT-549 (top) and ANV5 (bottom) cells. Data were normalized with absorbance values from day 0 and represent mean ± SD of six replicates. C. Percentage of BT549 (top) and ANV5 (bottom) cells in each phase of the cell cycle after maintaining cells in culture for 24 and 48 h. Sta, staurosporine. D. Percentage of apoptotic BT-549 (top) and ANV5 (bottom) cells in basal and staurosporine-induced conditions, measured by annexin-V binding flow cytometry assay. E. Quantification of spheres grown in 3D matrigel cultures. Data are mean ± SD of 8 replicates. Representative images at ×4 magnification. Scale bar 0.5 mm. F. Outline of the in vivo orthotopic experiment (n = 8 per group). G. Quantification of tumor volume at day 15 post-injection. H. Kaplan–Meier curves of resection-free survival. (PPTX 9850 kb)
Source: Figshare
Language: English
Topic: Cancer Cells and Metastasis
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7161381150