Work

In vitro and in silico study of the endosulfan degradation by Bacillus subtilis sp. strain UAMC

Year: 2026

Type: article

Abstract: The widespread use of endosulfan, a persistent organochlorine pesticide, has raised significant environmental and health concerns due to its toxicity and potential for bioaccumulation. This study investigated the degradation pathway of endosulfan by Bacillus subtilis sp. strain UAMC, combining in vitro and in silico approaches. These degradation assays revealed preferential degradation of α-endosulfan (97.6%) over β-endosulfan (69.45%), and identified key metabolites. The toxic endosulfan sulfate metabolite was not detected, suggesting a hydrolytic degradation mechanism. Molecular docking simulations highlighted interactions between endosulfan metabolites and enzymes such as laccase (3ZDW), which possess the necessary structural plasticity to recognize and transform endosulfan, even though they were not evolved for that specific purpose. These findings provided a mechanistic understanding of endosulfan biodegradation and underscore the potential of Bacillus subtilis sp. strain UAMC for pesticide bioremediation applications.

Source: Biodegradation

DOI: 10.1007/s10532-026-10312-y

Authors: Adriana Casanova, Sergio Hernández, Diego A. Esquivel-Hernández, Sergio Revah, Irmene Ortíz

Institution: Universidad Autónoma Metropolitana

Language: English

Topic: Pesticide and Herbicide Environmental Studies

Subfield: Pollution

Field: Environmental Science

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,590

Funder: Consejo Nacional de Ciencia y Tecnología