Work

Proteomic Identification of Small-Subunit Ribosome Assembly Factors in Trypanosoma brucei

Year: 2026

Type: article

Abstract: . We generated cell lines expressing tagged versions of UTP6 and PNO1, two conserved ribosome biogenesis factors that provide complementary access to complexes of early SSU processome intermediates and later pre-40S maturation stages. Affinity purification followed by mass spectrometry identified numerous conserved ribosome biogenesis factors alongside a substantial set of trypanosomatid-specific proteins with no assigned function. Structural analyses revealed that many of these uncharacterized proteins contain predicted RNA-binding motifs or protein-protein interaction domains, and have been previously localized to the nucleolus, supporting potential roles in ribosome synthesis. Our findings expand the repertoire of candidate SSU assembly factors in kinetoplastids and highlight species-specific adaptations in ribosome biogenesis, providing a foundation for future functional studies targeting these unique components.

Source: Journal of Proteome Research

DOI: 10.1021/acs.jproteome.6c00066

Authors: Gustavo Guadagnini Perez, Priscila Mazzocchi Hiraiwa, Verônica Santana da Silva, Nilson Ivo Tonin Zanchin, Beatriz Gomes Guimarães

Institutions: Fundação Oswaldo Cruz, Universidade Federal do Paraná

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,500

Funders: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico