Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles
Year: 2026
Type: article
Abstract: ABSTRACT The adult skeletal muscle regenerates robustly upon injury, but this regenerative capacity rapidly declines with age. In this study, we identify the lanthionine synthetase C-Like (LanCL) proteins, mammalian homologs of the bacterial peptide cyclase LanC, as positive regulators of muscle regeneration in middle-aged mice. In a barium chloride-induced injury model, we found the protein levels of LanCL1 and LanCL2 to increase during an early phase of regeneration in middle-aged (12-month-old) but not young adult (4-month-old) mice. Utilizing a mouse line lacking all three LanCL proteins (LanCL triple KO or LTKO), we examined a potential role of LanCL in injury-induced muscle regeneration. Consistent with an age-dependent function of LanCL, we observed a delayed regeneration of the tibialis anterior (TA) muscle after injury, as reflected by reduced regenerating myofiber sizes at day 7 after injury (D7AI) in middle-aged (but not young) LTKO compared to age-matched WT mice. The pool size of quiescent satellite cells (Pax7+) was comparable between 12-month-old LTKO and WT muscles without injury. However, the number of Pax7+ cells was significantly higher in regenerating LTKO muscles at both D3AI and D5AI, accompanied by reduced numbers of MyoD+ and MyoG+ cells. The percentage of proliferating Pax7+ cells was also higher in LTKO muscles at D5AI. Furthermore, we detected elevated expression of pro-inflammatory cytokines in regenerating LTKO muscles, while the number of macrophages was similar comparing LTKO and WT muscles. Taken together, our observations suggest that in aging muscles LanCLs are important for proper timing of inflammation resolution and regeneration upon injury. New & Noteworthy Physiological roles of the mammalian homologs of bacterial LanC, LanCLs, are poorly understood. Our work uncovers a function of LanCLs in post-injury regeneration of aging skeletal muscles. Middle-aged LanCL triple KO mice displayed a delay in satellite cell differentiation and regenerative myofiber formation, as well as persistent inflammatory cytokine expression, suggesting that LanCLs may have an age-dependent role in modulating inflammation in the injured muscles to facilitate regeneration.
Institution: University of Illinois Urbana-Champaign
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W7161736206