Additional file 3 of Targeting APE1 endonuclease activity impairs metastasis and enhances genotoxic therapy response in pancreatic cancer
Year: 2026
Type: other
Abstract: Supplementary Material 3. Supplemental Fig. 3: Knockdown of potential backup enzymes does not restore genotoxic sensitivity in E96A cells. (A) Effect of APEX2 knockdown. (Top) Representative images of methylene blue-stained colonies and corresponding binary segmentation masks for Cas9 control and E96A (clone B4) cells transfected with Scramble (SCR) or APEX2-targeting siRNA, followed by exposure to MMS (225µM, 30 min pulse). (Bottom Left) Validation of APEX2 knockdown by qPCR, showing relative APEX2 mRNA expression (ΔΔCt) following transfection with SCR or siAPEX2 in Cas9 and E96A (clone B4) cell lines (mean ± SEM). (Bottom Right) Colony area quantification (normalized to untreated SCR control for each cell line) is shown (mean ± SEM; n = 3). (B) Effect of PNKP knockdown. (Top) Representative images and segmentation masks for SCR and siPNKP transfected cells. (Bottom Left) Western blot validation of PNKP knockdown (55 kDa) with densitometric quantification. B4 and Cas9 cell lysates transfected with SCR or siPNKP were probed for PNKP and Vinculin (120 kDa; loading control). Data represent mean ± SEM; n = 3. (Bottom Right) Quantification of colony area. Data represent mean ± SEM; n = 3. No significant differences were observed between SCR and target-specific knockdown conditions within treatment groups (one-way ANOVA; p > 0.05).
Source: Figshare
Institution: Indiana University – Purdue University Indianapolis
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7161842410
Cites:
Cited by:
Related to: