Work

The Effective Biodegradation of Poly(ε-caprolactone) by Engineered Yeast Yarrowia lipolytica Producing Lipase B

Year: 2026

Type: article

Abstract: Poly(ε-caprolactone) (PCL) is a biodegradable aliphatic polyester with applications in many areas. Lipase B from Moesziomyces antarcticus (CALB, previously known as Candida antarctica lipase B) is a well-characterize enzyme capable of hydrolysing several polyesters. In this study, the codon-optimized gene encoding CALB was cloned into the yeast Yarrowia lipolytica to enhance its natural capabilities toward polyesters biodegradation. PCL films biodegradation was conducted directly in the medium using the engineered yeast at 28 °C. Process optimization employing baffled flasks significantly improved degradation efficiency and reduced time to 24 h. This study showed that the engineered yeast Y. lipolytica is a promising host for polyester biodegradation.

Source: International Journal of Molecular Sciences

DOI: 10.3390/ijms27104625

Authors: Żaneta Zdanowska, Lara Serrano-Aguirre, Aneta Krystyna Urbanek, Adam Dobrowolski, Aleksandra M. Mirończuk

Institutions: Consejo Superior de Investigaciones Científicas, Wrocław University of Environmental and Life Sciences, Centro de Investigaciones Biológicas Margarita Salas

Language: English

Topic: biodegradable polymer synthesis and properties

Subfield: Biomaterials

Field: Materials Science

Domain: Physical Sciences

SDG: Responsible consumption and production

Open Access status: gold

Estimated APC paid: $3,499