A 3D-printed extraction device based on aptamer-functionalized metal–organic frameworks for ochratoxin A determination in alcoholic beverages
Year: 2026
Type: article
Abstract: ABSTRACT This work reports the development of a 3D-printed extraction device coated with an aptamer-functionalized metal-organic framework (Apt@MOF) for the selective extraction of ochratoxin A (OTA) from alcoholic beverages. Several MOFs were initially screened under dispersive solid-phase extraction (dSPE) conditions, identifying UiO-66-(COOH) 2 as the most suitable support due to its minimal non-specific OTA retention, thus maximizing the contribution of Apt-based recognition. After selecting Apt immobilization through physical adsorption onto the MOF, the resulting hybrid material exhibited high selectivity toward OTA, being successfully immobilized onto polypropylene 3D-printed devices using a poly(vinylidene fluoride)-based coating. The optimized system provided high recoveries (91–95%), good reproducibility (RSD ≤ 5.5%), and a limit of detection of 0.06 μg L -1 , with a preconcentration factor of 4. The applicability of the method was demonstrated in wine and beer samples, showing efficient matrix clean-up and reliable OTA quantification. The combination of Apt-mediated recognition, MOF-supported immobilization, and 3D-printing versatility provides a practical, low-cost (∼0.49 € per device), and disposable alternative for selective OTA extraction from alcoholic beverages.
Source: Talanta
Institution: Universitat de València
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $4,610
OpenAlex ID: W7162311932