Space radiation induces distinct senescent phenotypes: Implications for space travel [scRNA-Seq]
Year: 2026
Type: dataset
Abstract: As Earth's magnetic field weakens, space radiation begins to pose a significant threat to the health of not only space travelers, but the world's population. Space radiation, comprising high-energy and high-charge ions, creates distinct clusters of DNA damage and dense macromolecular damage that result in the accumulation of senescent cells (SnCs) known to play a critical role in promoting multimorbidity. Here, we demonstrate that human fibroblasts exposed to different forms of space radiation acquire senescence-associated phenotypes including morphological alterations and the accumulation of SA-beta-gal+ cells to a greater extent than what is observed following gamma-irradiation. Bulk and single cell RNA (scRNAseq) sequencing analysis revealed that space irradiated human fibroblasts up-regulated senescent-like phenotypes to a greater extent compared to gamma-irradiation and enriched pathways associated with chronic activation and adaptation of the integrated stress response and NADPH-coupled redox metabolism. Healthy cells treated with conditioned media from irradiated SnCs manifested pro-inflammatory transcriptional profiles dependent on both radiation and cell type. Finally, treatment with known senotherapeutics demonstrated radiation-specific effects in primary dermal fibroblasts. Our data demonstrate that space radiation differentially induces senescent phenotypes in human cells compared to gamma-irradiation, which may play a key role in the pathogenic effects of space travel. This study hosts scRNAseq data, and the RNAseq data are available in OSD-993.
Source: NASA GeneLab
DOI: 10.26030/2rm9-3t98
Language: English
Subfield: Physiology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7162501016