Work

Two-Photon Microscopy in Biological Imaging: Current Uses and Evolving Potential

Year: 2026

Type: article

Abstract: Two-photon microscopy (TPM) has become an indispensable tool in the life sciences, offering exceptional spatial resolution, deep tissue penetration, and low phototoxicity. It provides a revolutionary solution for long-term dynamic observation of living cells and tissues. This article systematically explains the working principles of TPM and reviews its applications in cutting-edge fields such as neuroscience, immunology, oncology, regenerative science and plant biology, highlighting its remarkable multidisciplinary adaptability. Building on this foundation, we further explore the breakthrough directions and future prospects for the development of this technology.

Source: Photonics

DOI: 10.3390/photonics13060519

Authors: Qingpan Bu, Yinghui Gu, Xiaodan Wang, Shiwei Yuan, Kai Song, Xingyun Zhang

Institutions: Changchun Normal University, Chinese Academy of Sciences, Changchun Institute of Optics, Fine Mechanics and Physics

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,896

Funders: National Natural Science Foundation of China, Changchun Normal University