Year: 2026
Type: article
Abstract: Wheat blast, caused by the Magnaporthe oryzae Triticum pathotype (MoT), is a devastating fungal disease that poses a significant global threat to wheat production. Rapid diagnosis is crucial for effective monitoring, containment, and management of this highly transmissible pathogen. In this study, we developed an integrated field-detection kit, named W heat B last R apid D etection Kit (WB-RD kit), which utilizes recombinase polymerase amplification coupled with a nucleic acid lateral flow immunoassay (RPA–NALFIA). The kit is stable for room temperature transportation and achieves a limit of detection as low as 0.1 ng/μL for MoT genomic DNA. The RPA reaction only requires a constant temperature of 39°C for 10 min. The positive result is indicated by the appearance of both a control line and a test line on the lateral flow strip within 5 min. This rapid, precise, user-friendly, and portable method is suitable for both on‑site diagnosis and phytosanitary inspection of seed shipments, providing a practical tool for frontline disease monitoring and quarantine.
Source: Phytopathology Research
Authors: Ye Peng, Jianglin Xu, Batiseba Tembo, Xinyao He, Pawan Kumar Singh, Guo-Liang Wang, Houxiang Kang
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
SDG: Zero hunger
Open Access status: diamond
Awards: G5874933498, G7870951161, G8278199885
OpenAlex ID: W7162559787