Work

Microinjected dsRNA triggers a robust RNAi response in Stentor coeruleus

Year: 2026

Type: article

Abstract: Stentor coeruleus is an emerging model system for the study of morphogenesis and regeneration as well as behaviors like habituation and photosensation. While Stentor’s genome has been sequenced, genetic tools are currently limited to RNA interference by feeding. Here we show that microinjection of long double-stranded RNA is an effective method for triggering a robust RNAi response. Additionally, microinjection of mob1 dsRNA resulted in cell phenotypes appearing 24 hours earlier than by feeding bacterially expressed dsRNA. This work expands the genetic toolkit in Stentor when RNAi by feeding is not ideal for an experiment.

Source: PubMed Central

DOI: 10.17912/micropub.biology.002175

Authors: Makenna Kuecks, Preeti Arra, Sarah Hoffmann-Weitsman, Makenzie Funk Craig, Mark M Slabodnick

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W7162574829

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