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Screening Atg11, a central organizer of yeast selective autophagy, for residues involved in the Atg11-Atg9 interaction

Year: 2025

Type: article

Abstract: The yeast protein Atg11, whose structure is not fully solved, is a central organizer of autophagosome formation that recruits Atg9 during selective autophagy. Although the residues in Atg9 responsible for this interaction are known, those in Atg11 are not. In an attempt to discover the binding site of Atg9 on Atg11, we screened a number of mutants within amino acid residues 455-627 of Atg11, guided in part by an AlphaFold2-generated model of the Atg11 dimer. However, we were not able to identify specific residues essential for the interaction with Atg9, suggesting that the binding region may lie elsewhere on Atg11.

Source: PubMed

DOI: 10.17912/micropub.biology.001941

Authors: Chimi Dolker Sherpa, Patricia Woghiren, Erin Leonello, Mina Abdel-Khalek, Benjamin J. Schuessler, Josh V. Vermaas, Steven K. Backues

Language: English

Topic: Autophagy in Disease and Therapy

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

Open Access status: green

OpenAlex ID: W7162787986

FWCI:

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Related to: 10