Effects and mechanisms of mascRNA regulate the proliferation, migration and invasion of Laryngeal Squamous Cell Carcinoma in vitro
Year: 2026
Type: article
Abstract: OBJECTIVE: To explore the impact and mechanism of mascRNA on the proliferation, migration, and invasion of Laryngeal Squamous Cell Carcinoma. METHODS: Collecting clinical samples of LSCC and using qRT-PCR technology verified the differential expression of mascRNA in cancer tissues and adjacent tissues. Construct TU686 and TU177 cells with overexpression or knockdown mascRNA. Use qRT-PCR to verify their overexpression efficiency, colony formation assays, CCK-8 assays were performed to detect cell proliferation ability, tumor formation experiment in nude mice to detect the effect of mascRNA on tumor growth, scratch assays, transwell migration, and invasion assays to detect cell migration and invasion ability, Western blot assays to detect the expression of Epithelial-Mesenchymal Transition (EMT) related proteins. RESULT: We found that mascRNA was increasing in multiple clinical samples of laryngeal cancer tissue. Overexpressing mascRNA promotes the proliferation, migration, and invasion abilities of LSCC cells. Conversely, Knockdown mascRNA inhibits these abilities of LSCC cells. Furthermore, mascRNA enhances the tumor growth of LSCC. Moreover, we find that mascRNA overexpression promotes the expression of EMT-related proteins. CONCLUSION: Our study found that mascRNA enhances the proliferation, migratory, and invasive capabilities of LSCC.
Authors: Remark Jie Peng, Zengzhi Zhang, Sicheng Zhu, Yanqing Wu, Aoxuan Xu, Hui Li, Shiyin Ma, Xiaomin Wang
Institution: Bengbu Medical College
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,070
Funder: Bengbu Medical College
Awards: G2116526019, G8140545304, G8750140693
OpenAlex ID: W7162870977