Data from: SiglecF-expressing neutrophils differentiate from mature neutrophils locally in the heart after myocardial infarction
Year: 2026
Type: dataset
Abstract: Myocardial infarction (MI) triggers an immune response marked by rapid infiltration of neutrophils. Though once considered a uniform population, neutrophils display remarkable heterogeneity post-MI, including a subset expressing SiglecF, a marker typically associated with eosinophils. In this study, we investigated the origin of SiglecFPos neutrophils following MI. We found that these cells appear early in the heart after MI, coinciding with the influx of CD101Neg immature neutrophils. However, SiglecFPos neutrophils were absent in hematopoietic organs, suggesting their local differentiation. Interestingly, SiglecFPos neutrophils co-expressed CD101, indicating their maturity. Neutrophil stimulation in vitro with TGF-β and GM-CSF induced co-expression of SiglecF and CD101. When sorted CD101Neg and CD101Pos neutrophils were exposed to the same stimuli, only the mature (CD101Pos) subset could acquire SiglecF expression, indicating maturation as a prerequisite for SiglecF induction. Using EdU labeling prior to MI to track neutrophil origins in vivo, we found that most SiglecFPos neutrophils in infarcted hearts were derived from mature circulating neutrophils existing prior to MI. SiglecFPos neutrophils emerge during heightened inflammation, which drives emergency granulopoiesis in the bone marrow. Neutrophils from mice treated with G-CSF to stimulate emergency granulopoiesis exhibited enhanced capacity for SiglecF expression. Neutrophils exposed to G-CSF in vitro exhibited enhanced SiglecF induction by TGF-β, indicating that G-CSF primes neutrophils for SiglecF expression in vivo and ex vivo. These findings support a model in which SiglecFPos neutrophils originate from mature CD101Pos precursors following MI, and emergency granulopoiesis enhancing their capacity to acquire this phenotype and contributing to neutrophil heterogeneity in cardiac injury. Flow cytometry was the primary endpoint for each of these experiments.
Source: DRYAD
Authors: Stephan, Jonah, Knerr, Taylor, Isaacs, William, Nong, Yibing, Wysoczynski, Marcin
Institution: University of Louisville
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7163171019