Discovery of TCR-like antibodies to the KRAS G12D neoantigen via in silico-in vitro workflow

Year: 2026

Type: article

Abstract: /HLA-C∗08:02-specific antibodies with high affinity. Comprehensive specificity profiling using a phage display library confirmed the absence of human off-target reactivity, and in silico analysis predicted low immunogenicity. When reformatted as chimeric antigen receptors or bispecific T cell engagers, these antibodies mediated selective cytotoxicity against target-positive cells. Together, these findings establish a practical design-to-function pipeline for T cell receptor (TCR)-like antibody discovery and demonstrate the feasibility of therapeutically targeting KRAS G12D-driven malignancies.

Source: Molecular Therapy

DOI: 10.1016/j.ymthe.2026.05.032

Institutions: Korea Advanced Institute of Science and Technology, Korea Research Institute of Bioscience and Biotechnology, Osong Medical Innovation Foundation, Ajou University, Samsung Medical Center, Sungkyunkwan University, Cleveland Clinic

Language: English

Topic: Monoclonal and Polyclonal Antibodies Research

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,500

Funder: National Research Foundation of Korea

Awards: G1164878805, G7030625706

OpenAlex ID: W7163346980