Work

RcWRKY71 - RcGolS1 module enhances cold stress tolerance by oligosaccharides accumulation in rose ( Rosa chinensis )

Year: 2026

Type: article

Abstract: Succinct title This study identifies a novel regulatory module, RcWRKY71-RcGolS1, that enhances cold stress tolerance in Rosa chinensis by modulating oligosaccharide metabolism. Overexpression of RcWRKY71 improved cold resistance, as reflected in enhanced callus growth under low-temperature conditions. Conversely, silencing RcWRKY71 compromised cold tolerance, leading to reduced leaf photosynthetic capacity and decreased soluble sugar contents in roots. Additionally, we provide definitive evidence that RcWRKY71 directly binds to and transcriptionally activates its downstream target gene, RcGolS1. Suppression of RcGolS1 reduced cold tolerance, whereas its overexpression increased cold resistance via activation of raffinose family oligosaccharide biosynthesis, as indicated by elevated levels of myo-inositol, galactinol, and raffinose. Collectively, these findings elucidate a previously uncharacterized RcWRKY71-RcGolS1 regulatory pathway within the cold hardiness signaling network and offer critical molecular insights into the accumulation of cryoprotective oligosaccharides that contribute overwintering adaptation in perennial woody plants.

Source: Horticulture Research

DOI: 10.1093/hr/uhag218