Work

Generation of a STRAIGHT-IN Dual AAVS1 hiPSC line with orthogonal landing pads for versatile DNA payload integration

Year: 2026

Type: article

Abstract: The STRAIGHT-IN platform is designed for facile genomic integration of DNA payloads into human induced pluripotent stem cells (hiPSCs) that contain a pre-inserted landing pad (LP). Here, we expanded the versatility of STRAIGHT-IN by introducing an additional, orthogonal LP into the unmodified allele of the safe harbor locus AAVS1. Specifically, we targeted the hiPSC line LUMC0099iCTRL04_AAVS1-bxb-v2 (hPSCreg LUMCi004-A-1), which already carried one LP. The resulting STRAIGHT-IN AAVS1 Dual line can integrate two independent DNA payloads in parallel, expanding the applicability of the platform for complex genomic engineering applications.

Source: Stem Cell Research

DOI: 10.1016/j.scr.2026.104028

Authors: Babet van der Vaart, Albert Blanch-Asensio, Niels Geijsen, Christine L. Mummery, Christian Freund, Richard P. Davis

Institutions: Leiden University Medical Center, Novo Nordisk Foundation Center for Stem Cell Medicine

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,360

Funders: ZonMw, Nederlandse Organisatie voor Wetenschappelijk Onderzoek, Leids Universitair Medisch Centrum, Novo Nordisk Fonden