Work

Purification of ginsenoside glycosidase-BglPm using ITC of Elastin like protein-tagged system

Year: 2026

Type: article

Abstract: Rare ginsenosides are known to have many medicinal values such as anti-tumor and anti-oxidant activity.Rare ginsenosides can be obtained by hydrolyzing saponin glycosides in ginseng with ginsenoside glycosidase.Glycosidases with high purity and activity are necessary to increase the efficiency of saponin conversion.A good example of effective purification is to utilize the elastin-like polypeptide (ELP) system.This purification method has the advantages of high purity, low cost and simple operation.In this study, we optimized the purification conditions of BglPm-ELP by the inverse transition cycle (ITC) method.In addition, we also investigated the enzymatic characteristics of the enzyme.

Source: Drug Discovery

DOI: 10.54905/disssi.v20i45.e15dd3087

Authors: Hyok-Chol Ri, Jong-Hyok Kim, Chang-Bong Choe, Gyong- Jin Mun, Il-Tae Kim, Shu-Yang Dang, Su-Chol Rim, Kwang-Il To

Institutions: Pyongyang University of Science and Technology, Pyongyang Medical University, Northeast Forestry University

Language: English

Topic: Enzyme Production and Characterization

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

OpenAlex ID: W7163955854

FWCI:

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