Establishment of Embryogenic Cell Suspension Culture and Plant Regeneration of Rare Endangered Plant Emmenopterys henryi
Year: 2008
Type: article
Abstract: Embryogenic cell suspension cultures were established from calli of immature seeds of Emmenopterys henryi. Callus could be obtained from immature seeds on MS solid medium augmented with 2.0 mg·L-16-benzylaminopurine (6-BA) and 3% sucrose. Embryogenic calli were induced on MS basal medium supplemented with 0.1 mg·L-1 N-acetyl aspartate (NAA) and 0.5 mg ·L-16-BA. Numerous somatic embryoids appeared on MS liquid basal nutrient medium with 0.5 mg·L-1 NAA and 0.5 mg·L-16-BA. The optimal value of the initial cell density was 2.0% (fresh weight). Several parameters that might be associated with growth were determined over a 14-day period. The embryo-derived plantlets with well-developed roots and shoots were transferred successfully to the greenhouse, with a high survival rate of 95%.
Source: Chinese Bulletin of Botany
DOI: 10.3724/cbb-t27-105
Authors: Dan Xiong, Faju Chen, Hongwei Liang, Yubing Wang
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W7164040805