Figure 2 from Targeting GSTZ1 Sensitizes KRASG12C-Mutant Lung Cancer Cells by Overcoming Glutathione and Glycolysis Pathway Rewiring
Year: 2026
Type: other
Abstract: GSTZ1 depletion induces metabolic alterations in KRASG12C-mutant NSCLC. A, Effects of GSTZ1 silencing (red circles), sotorasib (Soto) treatment (blue circles) or their combination (red/blue semicircles) on glycolysis and glutamine metabolism. Solid circles in volcano blots, in contrast to empty circles, represent significant changes in signals that pass both the significance and log2 ratio cutoffs, shown by dashed lines. Lines and arrows denote direct or indirect interactions. The ratio reflects the metabolite signal of each treatment compared with the siNT control. Glyceraldehyde 3-phosphate is abbreviated as G3P. B, Immunoblots of GSTZ1 and actin. C–E, Volcano plots showing significantly altered annotated metabolites (orange) in H1792 cells following the treatment with siGSTZ1, Soto (1 μmol/L), or combination treatment vs. control. Significance cutoffs were applied: log2 ratio ±0.585 and P < 0.05, and selected metabolites were highlighted. F, Bar plots of relative metabolite abundance of lactate, glutamine, GSSG, and the ratio of GSSG to GSH. Lactate, glutamine, GSSG, and GSH signals were extracted from C–E. G, Enriched metabolic pathways based on significantly altered metabolites with GSH and glycolysis pathways highlighted. Statistical analysis was conducted using Welch t test. Mean ± SD shown for four replicates (N = 4). *, P < 0.05; **, P < 0.01; ***, P < 0.001; n.s., not significant.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W7164315876