Figure 3 from Targeting GSTZ1 Sensitizes KRASG12C-Mutant Lung Cancer Cells by Overcoming Glutathione and Glycolysis Pathway Rewiring
Year: 2026
Type: other
Abstract: GSTZ1 knockdown impairs redox balance and sensitizes NSCLC cells to oxidative stress. A, Relative GSH and (B) lactate levels in H1792 and HOP62 cells following siGSTZ1 or BSO (500 μmol/L) treatment for 48 hours. GSTZ1 knockdown was confirmed by immunoblotting. N = 3. C, Images of ROS signals via H2DCFH-DA fluorescence in H1792 cells following treatment with siGSTZ1, N-acetyl cysteine (NAC, 2 mmol/L), and their combination for 48 hours. H2DCFH-DA fluorescence intensity was measured using a microplate reader at the excitation 485 nm and emission 530 nm. N = 3. D, Viability of H1792 cells treated with siGSTZ1, sotorasib (Soto; 1 μmol/L), and the antioxidant Trolox (50 μmol/L) for 96 hours. Cell viability was measured using CellTiter-Glo luminescent cell viability assay. N = 3. Welch t test was applied for the comparison of lactate levels in H1792 and HOP62 cells. Other data were analyzed by one-way ANOVA with Tukey multiple comparisons test. Data represent the mean ± SD.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W7164412889