Antibiofilm activity of zinc oxide nanoparticles on multidrug-resistant clinical isolates of Pseudomonas aeruginosa
Year: 2026
Type: article
Abstract: Aims: This study examines the antibiofilm activity of zinc oxide nanoparticles (ZnO NPs) against multidrug-resistant Pseudomonas aeruginosa isolates.Pseudomonas aeruginosa is a leading cause of morbidity and mortality in burn patients and immunocompromised individuals.This study was aimed to investigate the effects of ZnO NPs on biofilm formation and eradication.The study involved analyzing clinical samples collected from 120 patients in Baghdad. Methodology and results:This study included 120 clinical specimens collected from patients with different infections from four hospitals in Baghdad.Morphological and biochemical tests were performed on the bacterial isolates.The antibiotic susceptibility was evaluated using the disc diffusion method, while the microtiter plate method evaluated the biofilm formation.Of all the samples analyzed, 55 isolates (45.8%) were identified as P. aeruginosa.Among these isolates, 10 (18.2%) were found to be strong biofilm producers.The study revealed a significant reduction in biofilm formation after adding ZnO NPs compared to pre-treatment levels (p <0.01).Pseudomonas aeruginosa isolates displayed high resistance to levofloxacin, ceftazidime, imipenem, tobramycin and aztreonam.Conclusion, significance and impact of study: The observed significant antibiofilm effect on the multidrug-resistant isolates underscores the potential clinical application of ZnO NPs while emphasizing the imperative for additional validation of their therapeutic effectiveness and safety.
DOI: 10.21161/mjm.230337
Authors: Maala, A. S., Tutar, L., Ghaima, K. K.
Institutions: Ahi Evran University, University of Baghdad
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $110
OpenAlex ID: W7164749712