Effect of Early Weaning and Supplementation on the Longissimus thoracis Muscle Proteome of Pasture-Raised Nellore Bulls
Year: 2026
Type: article
Abstract: High Resolution Image Download MS PowerPoint Slide The aim of this study was to compare the Longissimus thoracis (LT) muscle proteomes of Nellore bulls subjected to early weaning (EW; 120 days, n = 20) or conventional weaning (CW; 205 days, n = 20). From 120 to 205 days of age, EW calves received protein−energy supplementation (10 g/kg body weight/day), whereas CW calves remained with their dams and received mineral supplementation. After 205 days, all animals were raised together on pasture with protein−energy supplementation until slaughter (915 days). LT biopsies were collected at 205 and 915 days and analyzed by nanoLC-MS/MS. At 205 days, EW calves showed lower abundances of proteins related to β-oxidation and heat shock response, and higher abundance of proteins related to muscle growth, than CW calves. At slaughter, fewer differences were observed between treatments, and the enriched pathways were primarily associated with oxidative phosphorylation. Overall, EW with supplementation altered proteins related to lipid metabolism at 205 days; however, these differences were not maintained until slaughter.
Authors: Juliana Akamine Torrecilhas, Gustavo Henrique Russo, Gustavo Lucas Bezerra Tinoco, Paloma Leandra Garcia Melo, Philipe Moriel, Hugo Osório, David Miguel Ribeiro, Welder Angelo Baldassini, Rogerio Abdalla Curi, André Martinho de Almeida, Otávio Rodrigues Machado Neto, Guilherme Luís Pereira, Luís Artur Loyola Chardulo
Language: English
Subfield: Agronomy and Crop Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
Estimated APC paid: $4,500
Awards: Global gene expression and epigenetic changes in Nellore cattle submitted to different weaning protocols and their effect on carcass and meat quality, G2125541387, G3023625416, Laboratório para a Sustentabilidade do Uso da Terra e dos Serviços dos Ecossistemas, G3232384975, Laboratório Associado para Ciência Animal e Veterinária, G3636998759, G5853743737, LEAF - Linking Landscape, Environment, Agriculture and Food, Centro de Investigação Interdisciplinar em Sanidade Animal
OpenAlex ID: W7164886773