Supplemental Figure S11 from VAX014 Activates Tumor-Intrinsic STING and RIG-I to Promote the Development of Antitumor Immunity
Year: 2026
Type: supplementary-materials
Abstract: Supplemental Figure S11. In vitro confirmation of all MB49 RIG-I KO clones and verification of intact STING activity. A) Expression of STING and RIG-I in MB49WT (WT) and MB49RIG-I KO (RIG-I KO) clones, using Vinculin or GAPDH as a loading control, representative of at least two independent experiments. B-C) Cells were plated (5.0x105 cells/well) and treated with DMXAA (30 μg/mL) ± H-151 (4 μg/mL) for ∼20 hours. B) IFN-β in supernatants of treated cells as measured via ELISA (n= 3). C) Surface expression of PD-L1 and MHC-I in cells as determined by flow cytometry (n= 3). Histograms depict mean ± SEM of all combined experiments and dashed lines represent the baseline expression value of the corresponding untreated control. #indicates which RIG-I KO clone is represented in Figure 4.
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W7165002075