Year: 2026
Type: article
Abstract: In this narrative review, the systematic tailoring of cell-derived secretomes for regenerative medicine is comprehensively discussed. The review begins by evaluating a diverse array of cell sources, including platelets, endothelial cells, osteoblasts, chondrocytes, tenocytes, and mesenchymal stem cells (MSCs). From this foundation, we critically analyze how modulating cell culture conditions can be used to directly influence the therapeutic and paracrine profiles of the resulting secretomes. Specifically, we address the impact of the following critical parameters on secretome fabrication: (i) Culture Medium Supplementation: The use of defined media, supplements, and chemical priming agents. (ii) Cell Cultivation Formats: A comparative analysis of 2D monolayer cultures versus 3D spheroids. (iii) Culture Duration and Fluidics: The effects of culture time alongside static versus dynamic culture systems. (iv) Microenvironmental Stress: The influence of varying oxygen levels (for example, hypoxia) and induced oxidative stress on secretory output.
Source: Biology
Author: Johanna Buschmann
Institution: University Hospital Zurich
Language: English
Subfield: Urology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,258
OpenAlex ID: W7165010015